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human protein kinase library  (Addgene inc)


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    Addgene inc human protein kinase library
    <t>Human</t> <t>protein</t> <t>kinase</t> <t>library</t> screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.
    Human Protein Kinase Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human protein kinase library/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    human protein kinase library - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "CRISPR/Cas9 Screening Highlights PFKFB3 Gene as a Major Contributor to 5-Fluorouracil Resistance in Esophageal Cancer"

    Article Title: CRISPR/Cas9 Screening Highlights PFKFB3 Gene as a Major Contributor to 5-Fluorouracil Resistance in Esophageal Cancer

    Journal: Cancers

    doi: 10.3390/cancers17101637

    Human protein kinase library screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.
    Figure Legend Snippet: Human protein kinase library screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.

    Techniques Used: Knock-Out, Generated, CRISPR, Western Blot, Gene Knockout



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    <t>Human</t> <t>protein</t> <t>kinase</t> <t>library</t> screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.
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    <t>Human</t> <t>protein</t> <t>kinase</t> <t>library</t> screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.
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    Image Search Results


    Human protein kinase library screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.

    Journal: Cancers

    Article Title: CRISPR/Cas9 Screening Highlights PFKFB3 Gene as a Major Contributor to 5-Fluorouracil Resistance in Esophageal Cancer

    doi: 10.3390/cancers17101637

    Figure Lengend Snippet: Human protein kinase library screen identified the PFKFB3 gene involved in esophageal cancer chemoresistance ( A ) Schematic outline of 5-FU drug resistance screening. ( B ) Identification of good sgRNA using MAGeCK-VISPR analysis. ( C – E ) PFKFB3 knockout KYSE-70, KYSE-270, and KYSE-150 cell lines were generated using CRISPR/Cas9 technology, and Western blot analysis was employed to confirm the efficiency of the PFKFB3 gene knockout.

    Article Snippet: The human protein kinase library, which was a gift from John Doench and David Root (RRID: Addgene_75312), and additional sgRNAs designed to target genes involved in protein kinase [ ] contained 6934 sgRNAs targeting 1053 human genes and 107 NTCs (non-targeting-controls).

    Techniques: Knock-Out, Generated, CRISPR, Western Blot, Gene Knockout